Development of pcr method for Salmonella Enterica dna identification
نویسندگان
چکیده
منابع مشابه
An optimized affordable DNA-extraction method from Salmonella enterica Enteritidis for PCR experiments
In diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. If not inactivated properly, cellular and/or environmental DNA nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in PCR experiments. When crude cell extracts, proteinase K–treat...
متن کاملan optimized affordable dna-extraction method from salmonella enterica enteritidis for pcr experiments
in diagnostic and research bacteriology settings with budget and staff restrictions, fast and cost-effective genome extraction methods are desirable. if not inactivated properly, cellular and/or environmental dna nucleases will degrade genomic material during the extraction stage, and therefore might give rise to incorrect results in pcr experiments. when crude cell extracts, proteinase k–treat...
متن کاملA Multiplex PCR based Method for the Identification of common Clinical Serotypes of Salmonella enterica subspecies enterica
A multiplex PCR method has been developed to differentiate between the most common clinical serotypes of Salmonella enterica subsp. enterica encountered in Washington State and the USA in general. Six genetic loci from S. Typhimurium and four from S. Typhi were used to create an assay consisting of two five-plex PCR reactions. The assays gave reproducible results with 30 different serotypes tha...
متن کاملidentification of isolated salmonella enterica serotype gallinarum biotype pullorum and gallinarum by pcr-rflp
background salmonella spp. is the major bacterial pathogen in poultry and is responsible for significant economic losses of the poultry industry in many parts of the world. among salmonella spp., salmonella gallinarum and s. pullorum are the most common causative agents of chicken salmonellosis resulting in high mortality and morbidity. objectives the aim of this study was to identify s. gallin...
متن کاملPotential Use of DNA Aptamer-Magnetic Bead Separation-PCR Assay for Salmonella Detection in Food
Background: Salmonella is one of the most common food-borne pathogens that can cause illness. In this study, the sensitivity and the specificity of Aptamer-Magnetic bead Separation-Polymerase Chain Reaction (AMS-PCR) method were determined for Salmonella spp. detection. Methods: Different concentrations of Salmonella enterica were mixed with streptavidin-magnetic beads coated with biotinylated...
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ژورنال
عنوان ژورنال: Experimental Biology
سال: 2020
ISSN: 1563-0218,2617-7498
DOI: 10.26577//eb.2020.v83.i2.10